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primary antibodies against fibulin 3 efemp1  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology primary antibodies against fibulin 3 efemp1
    Representative microphotographs showing elastofibrosis of the muscularis propria and subserosa associated with <t>EFEMP1</t> deposition. ( a – h ) Colon: ( a , b , e , f ) elastica–Masson staining; ( c , g ) Congo red staining; ( d , h ) IHC for EFEMP1. ( a – d ) 31 colon cases; ( e – h ) 37 colon cases. Panels ( b – d ) and panels ( f – h ) show the same sites in serial sections, respectively. ( a – d ) Elastofibrosis is observed in the outer part of the longitudinal muscular layer to the superficial part of the subserosa ( a , b ). In this lesion, congophilia was nearly negative, whereas obvious immunoreactivity for EFEMP1 was identified. ( e – h ) Proliferation of elastic fibers is obvious compared with the first case ( e , f ). However, congophilia and immunoreactivity for EFEMP1 are similar to the first case ( g , h ). Scale bar = 200 μm ( a , e ); 100 μm ( b – d , f – h ).
    Primary Antibodies Against Fibulin 3 Efemp1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+fibulin+3+efemp1/Fibulin-3+Antibody/pmc11277079-172-5-14
    Average 93 stars, based on 47 article reviews
    primary antibodies against fibulin 3 efemp1 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Clinicopathological Appearance of Epidermal Growth-Factor-Containing Fibulin-like Extracellular Matrix Protein 1 Deposition in the Lower Gastrointestinal Tract: An Autopsy-Based Study"

    Article Title: Clinicopathological Appearance of Epidermal Growth-Factor-Containing Fibulin-like Extracellular Matrix Protein 1 Deposition in the Lower Gastrointestinal Tract: An Autopsy-Based Study

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25147581

    Representative microphotographs showing elastofibrosis of the muscularis propria and subserosa associated with EFEMP1 deposition. ( a – h ) Colon: ( a , b , e , f ) elastica–Masson staining; ( c , g ) Congo red staining; ( d , h ) IHC for EFEMP1. ( a – d ) 31 colon cases; ( e – h ) 37 colon cases. Panels ( b – d ) and panels ( f – h ) show the same sites in serial sections, respectively. ( a – d ) Elastofibrosis is observed in the outer part of the longitudinal muscular layer to the superficial part of the subserosa ( a , b ). In this lesion, congophilia was nearly negative, whereas obvious immunoreactivity for EFEMP1 was identified. ( e – h ) Proliferation of elastic fibers is obvious compared with the first case ( e , f ). However, congophilia and immunoreactivity for EFEMP1 are similar to the first case ( g , h ). Scale bar = 200 μm ( a , e ); 100 μm ( b – d , f – h ).
    Figure Legend Snippet: Representative microphotographs showing elastofibrosis of the muscularis propria and subserosa associated with EFEMP1 deposition. ( a – h ) Colon: ( a , b , e , f ) elastica–Masson staining; ( c , g ) Congo red staining; ( d , h ) IHC for EFEMP1. ( a – d ) 31 colon cases; ( e – h ) 37 colon cases. Panels ( b – d ) and panels ( f – h ) show the same sites in serial sections, respectively. ( a – d ) Elastofibrosis is observed in the outer part of the longitudinal muscular layer to the superficial part of the subserosa ( a , b ). In this lesion, congophilia was nearly negative, whereas obvious immunoreactivity for EFEMP1 was identified. ( e – h ) Proliferation of elastic fibers is obvious compared with the first case ( e , f ). However, congophilia and immunoreactivity for EFEMP1 are similar to the first case ( g , h ). Scale bar = 200 μm ( a , e ); 100 μm ( b – d , f – h ).

    Techniques Used: Staining

    Representative microphotographs of ATTR and EFEMP1 deposition. ( a – f ) Small intestine; ( g , h ) colon. ( a , c , d ) Congo red staining under bright-field illumination ( a , c ) and under polarized light ( d ); (panel a , inset) IHC for transthyretin; ( e – h ) double IHC for transthyretin and EFEMP1. ( a , b ) ATTR deposition pattern in the submucosa. ATTR deposition is identified mainly in the vessels and involves relatively small arteries (arrowheads in panel b ). Involvement of the veins (arrows in panel b ) is not obvious. ( c , d ) ATTR deposition pattern in the subserosa. ATTR deposition (arrowhead) shows stronger congophilia than EFEMP1 deposition (arrow) and exhibits clear apple-green birefringence under polarized light (arrowhead in panel d ). ( e , f ) Most ATTR and EFEMP1/AEFEMP1 deposits are observed independently (arrowheads and arrows indicate depositions of ATTR and EFEMP1/AEFEMP1, respectively). ( g , h ) However, colocalization of the two deposition types is identified in some vessels (arrowhead indicates ATTR deposition; arrow indicates ATTR and EFEMP1/AEFEMP1 deposition). Scale bar = 200 μm ( a , e ); 100 μm ( b – d , f – h ).
    Figure Legend Snippet: Representative microphotographs of ATTR and EFEMP1 deposition. ( a – f ) Small intestine; ( g , h ) colon. ( a , c , d ) Congo red staining under bright-field illumination ( a , c ) and under polarized light ( d ); (panel a , inset) IHC for transthyretin; ( e – h ) double IHC for transthyretin and EFEMP1. ( a , b ) ATTR deposition pattern in the submucosa. ATTR deposition is identified mainly in the vessels and involves relatively small arteries (arrowheads in panel b ). Involvement of the veins (arrows in panel b ) is not obvious. ( c , d ) ATTR deposition pattern in the subserosa. ATTR deposition (arrowhead) shows stronger congophilia than EFEMP1 deposition (arrow) and exhibits clear apple-green birefringence under polarized light (arrowhead in panel d ). ( e , f ) Most ATTR and EFEMP1/AEFEMP1 deposits are observed independently (arrowheads and arrows indicate depositions of ATTR and EFEMP1/AEFEMP1, respectively). ( g , h ) However, colocalization of the two deposition types is identified in some vessels (arrowhead indicates ATTR deposition; arrow indicates ATTR and EFEMP1/AEFEMP1 deposition). Scale bar = 200 μm ( a , e ); 100 μm ( b – d , f – h ).

    Techniques Used: Staining

    Summary of clinical and pathological data in patients with definite or possible constipation.
    Figure Legend Snippet: Summary of clinical and pathological data in patients with definite or possible constipation.

    Techniques Used:

    Schematic presentation of the hypothesized pathological effects of EFEMP1/AEFEMP1 deposition in the lower gastrointestinal tract. It is hypothesized that EFEMP1/AEFEMP1 deposition in blood vessels causes impaired blood flow, whereas deposition around the Auerbach plexus, muscularis propria, subserosa, and serosa reduces peristalsis and causes wall vulnerability. Complications such as hypothyroidism, LBD, and diabetes are presumed to be exacerbating factors in these conditions. A combination of these pathologies may result in ischemia of the intestinal tract and associated perforation, ileus, intestinal volvulus, constipation, and diverticulum formation.
    Figure Legend Snippet: Schematic presentation of the hypothesized pathological effects of EFEMP1/AEFEMP1 deposition in the lower gastrointestinal tract. It is hypothesized that EFEMP1/AEFEMP1 deposition in blood vessels causes impaired blood flow, whereas deposition around the Auerbach plexus, muscularis propria, subserosa, and serosa reduces peristalsis and causes wall vulnerability. Complications such as hypothyroidism, LBD, and diabetes are presumed to be exacerbating factors in these conditions. A combination of these pathologies may result in ischemia of the intestinal tract and associated perforation, ileus, intestinal volvulus, constipation, and diverticulum formation.

    Techniques Used:

    Representative microphotographs of EFEMP1 on elastica–Masson and Congo red staining and IHC for EFEMP1 in the lower gastrointestinal tract. Deposits in the vessel ( a – c ); mucosa and muscularis mucosae ( d – f ); muscularis propria ( g – i ); Auerbach plexus ( j – l ); serosa ( m – o ); and interstitium of the subserosa (including the mesentery) ( p – r ). ( a , d , g , j , m , p ) Elastica–Masson staining; ( b , e , h , k , n , q ) Congo red staining under bright-field illumination; (insets in ( b , n )) Congo red staining under polarized light; ( c , f , i , l , o , r ) IHC for EFEMP1. Notably, areas positive for EFEMP1 deposition show proliferation (panel d ), contortion, thickening, and fragmentation of elastic fibers (panels a , g , j , m , p ). Deposits in the muscularis mucosae show no congophilia ( e ), and deposits in the muscularis propria ( h ), peri-Auerbach plexus ( k ), and interstitium of the subserosa ( q ) exhibit weak congophilia compared with that in the vessel ( b ) and serosa ( n ). Arrowheads indicate EFEMP1 deposits, and the area indicated by the black square is the region shown in the inset. Scale bar = 200 μm ( d – f , g – i ); 100 μm ( a – c , g – i , m – r ).
    Figure Legend Snippet: Representative microphotographs of EFEMP1 on elastica–Masson and Congo red staining and IHC for EFEMP1 in the lower gastrointestinal tract. Deposits in the vessel ( a – c ); mucosa and muscularis mucosae ( d – f ); muscularis propria ( g – i ); Auerbach plexus ( j – l ); serosa ( m – o ); and interstitium of the subserosa (including the mesentery) ( p – r ). ( a , d , g , j , m , p ) Elastica–Masson staining; ( b , e , h , k , n , q ) Congo red staining under bright-field illumination; (insets in ( b , n )) Congo red staining under polarized light; ( c , f , i , l , o , r ) IHC for EFEMP1. Notably, areas positive for EFEMP1 deposition show proliferation (panel d ), contortion, thickening, and fragmentation of elastic fibers (panels a , g , j , m , p ). Deposits in the muscularis mucosae show no congophilia ( e ), and deposits in the muscularis propria ( h ), peri-Auerbach plexus ( k ), and interstitium of the subserosa ( q ) exhibit weak congophilia compared with that in the vessel ( b ) and serosa ( n ). Arrowheads indicate EFEMP1 deposits, and the area indicated by the black square is the region shown in the inset. Scale bar = 200 μm ( d – f , g – i ); 100 μm ( a – c , g – i , m – r ).

    Techniques Used: Staining

    Representative microphotographs of EFEMP1 deposition grading used in this study: ( a – n ) IHC for EFEMP1; ( a , f , k ) vessel; ( b , g ) muscularis propria; ( c , h , l ) Auerbach plexus; ( d , i , m ) serosa; and ( e , j , n ) mesentery (interstitium). Arrowheads indicate EFEMP1 deposits. Scale bar = 200 μm ( a – n ).
    Figure Legend Snippet: Representative microphotographs of EFEMP1 deposition grading used in this study: ( a – n ) IHC for EFEMP1; ( a , f , k ) vessel; ( b , g ) muscularis propria; ( c , h , l ) Auerbach plexus; ( d , i , m ) serosa; and ( e , j , n ) mesentery (interstitium). Arrowheads indicate EFEMP1 deposits. Scale bar = 200 μm ( a – n ).

    Techniques Used:

    Related Articles

    Immunohistochemistry:

    Article Title: Clinicopathological Appearance of Epidermal Growth-Factor-Containing Fibulin-like Extracellular Matrix Protein 1 Deposition in the Lower Gastrointestinal Tract: An Autopsy-Based Study
    Article Snippet: .. Single IHC was performed using primary antibodies against fibulin-3 (EFEMP1) (mouse, clone mab3-5, 1:2000, Santa Cruz, TX, USA), and phosphorylated α-synuclein (clone LB508, 1:500; Zymed, San Francisco, CA, USA) was performed in all cases. .. IHC was performed for prealbumin (transthyretin) (rabbit, clone EPR3219, 1:2000, Abcam, Cambridge, UK) in cases with positive ATTR-CA and a deposition pattern suspicious of ATTR deposition in the intestine and/or colon on Congo red-stained specimens.



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    primary antibodies against fibulin 3 efemp1  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology primary antibodies against fibulin 3 efemp1
    Representative microphotographs showing elastofibrosis of the muscularis propria and subserosa associated with <t>EFEMP1</t> deposition. ( a – h ) Colon: ( a , b , e , f ) elastica–Masson staining; ( c , g ) Congo red staining; ( d , h ) IHC for EFEMP1. ( a – d ) 31 colon cases; ( e – h ) 37 colon cases. Panels ( b – d ) and panels ( f – h ) show the same sites in serial sections, respectively. ( a – d ) Elastofibrosis is observed in the outer part of the longitudinal muscular layer to the superficial part of the subserosa ( a , b ). In this lesion, congophilia was nearly negative, whereas obvious immunoreactivity for EFEMP1 was identified. ( e – h ) Proliferation of elastic fibers is obvious compared with the first case ( e , f ). However, congophilia and immunoreactivity for EFEMP1 are similar to the first case ( g , h ). Scale bar = 200 μm ( a , e ); 100 μm ( b – d , f – h ).
    Primary Antibodies Against Fibulin 3 Efemp1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+fibulin+3+efemp1/Fibulin-3+Antibody/pmc11277079-172-5-14
    Average 93 stars, based on 1 article reviews
    primary antibodies against fibulin 3 efemp1 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

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    Representative microphotographs showing elastofibrosis of the muscularis propria and subserosa associated with EFEMP1 deposition. ( a – h ) Colon: ( a , b , e , f ) elastica–Masson staining; ( c , g ) Congo red staining; ( d , h ) IHC for EFEMP1. ( a – d ) 31 colon cases; ( e – h ) 37 colon cases. Panels ( b – d ) and panels ( f – h ) show the same sites in serial sections, respectively. ( a – d ) Elastofibrosis is observed in the outer part of the longitudinal muscular layer to the superficial part of the subserosa ( a , b ). In this lesion, congophilia was nearly negative, whereas obvious immunoreactivity for EFEMP1 was identified. ( e – h ) Proliferation of elastic fibers is obvious compared with the first case ( e , f ). However, congophilia and immunoreactivity for EFEMP1 are similar to the first case ( g , h ). Scale bar = 200 μm ( a , e ); 100 μm ( b – d , f – h ).

    Journal: International Journal of Molecular Sciences

    Article Title: Clinicopathological Appearance of Epidermal Growth-Factor-Containing Fibulin-like Extracellular Matrix Protein 1 Deposition in the Lower Gastrointestinal Tract: An Autopsy-Based Study

    doi: 10.3390/ijms25147581

    Figure Lengend Snippet: Representative microphotographs showing elastofibrosis of the muscularis propria and subserosa associated with EFEMP1 deposition. ( a – h ) Colon: ( a , b , e , f ) elastica–Masson staining; ( c , g ) Congo red staining; ( d , h ) IHC for EFEMP1. ( a – d ) 31 colon cases; ( e – h ) 37 colon cases. Panels ( b – d ) and panels ( f – h ) show the same sites in serial sections, respectively. ( a – d ) Elastofibrosis is observed in the outer part of the longitudinal muscular layer to the superficial part of the subserosa ( a , b ). In this lesion, congophilia was nearly negative, whereas obvious immunoreactivity for EFEMP1 was identified. ( e – h ) Proliferation of elastic fibers is obvious compared with the first case ( e , f ). However, congophilia and immunoreactivity for EFEMP1 are similar to the first case ( g , h ). Scale bar = 200 μm ( a , e ); 100 μm ( b – d , f – h ).

    Article Snippet: Single IHC was performed using primary antibodies against fibulin-3 (EFEMP1) (mouse, clone mab3-5, 1:2000, Santa Cruz, TX, USA), and phosphorylated α-synuclein (clone LB508, 1:500; Zymed, San Francisco, CA, USA) was performed in all cases.

    Techniques: Staining

    Representative microphotographs of ATTR and EFEMP1 deposition. ( a – f ) Small intestine; ( g , h ) colon. ( a , c , d ) Congo red staining under bright-field illumination ( a , c ) and under polarized light ( d ); (panel a , inset) IHC for transthyretin; ( e – h ) double IHC for transthyretin and EFEMP1. ( a , b ) ATTR deposition pattern in the submucosa. ATTR deposition is identified mainly in the vessels and involves relatively small arteries (arrowheads in panel b ). Involvement of the veins (arrows in panel b ) is not obvious. ( c , d ) ATTR deposition pattern in the subserosa. ATTR deposition (arrowhead) shows stronger congophilia than EFEMP1 deposition (arrow) and exhibits clear apple-green birefringence under polarized light (arrowhead in panel d ). ( e , f ) Most ATTR and EFEMP1/AEFEMP1 deposits are observed independently (arrowheads and arrows indicate depositions of ATTR and EFEMP1/AEFEMP1, respectively). ( g , h ) However, colocalization of the two deposition types is identified in some vessels (arrowhead indicates ATTR deposition; arrow indicates ATTR and EFEMP1/AEFEMP1 deposition). Scale bar = 200 μm ( a , e ); 100 μm ( b – d , f – h ).

    Journal: International Journal of Molecular Sciences

    Article Title: Clinicopathological Appearance of Epidermal Growth-Factor-Containing Fibulin-like Extracellular Matrix Protein 1 Deposition in the Lower Gastrointestinal Tract: An Autopsy-Based Study

    doi: 10.3390/ijms25147581

    Figure Lengend Snippet: Representative microphotographs of ATTR and EFEMP1 deposition. ( a – f ) Small intestine; ( g , h ) colon. ( a , c , d ) Congo red staining under bright-field illumination ( a , c ) and under polarized light ( d ); (panel a , inset) IHC for transthyretin; ( e – h ) double IHC for transthyretin and EFEMP1. ( a , b ) ATTR deposition pattern in the submucosa. ATTR deposition is identified mainly in the vessels and involves relatively small arteries (arrowheads in panel b ). Involvement of the veins (arrows in panel b ) is not obvious. ( c , d ) ATTR deposition pattern in the subserosa. ATTR deposition (arrowhead) shows stronger congophilia than EFEMP1 deposition (arrow) and exhibits clear apple-green birefringence under polarized light (arrowhead in panel d ). ( e , f ) Most ATTR and EFEMP1/AEFEMP1 deposits are observed independently (arrowheads and arrows indicate depositions of ATTR and EFEMP1/AEFEMP1, respectively). ( g , h ) However, colocalization of the two deposition types is identified in some vessels (arrowhead indicates ATTR deposition; arrow indicates ATTR and EFEMP1/AEFEMP1 deposition). Scale bar = 200 μm ( a , e ); 100 μm ( b – d , f – h ).

    Article Snippet: Single IHC was performed using primary antibodies against fibulin-3 (EFEMP1) (mouse, clone mab3-5, 1:2000, Santa Cruz, TX, USA), and phosphorylated α-synuclein (clone LB508, 1:500; Zymed, San Francisco, CA, USA) was performed in all cases.

    Techniques: Staining

    Summary of clinical and pathological data in patients with definite or possible constipation.

    Journal: International Journal of Molecular Sciences

    Article Title: Clinicopathological Appearance of Epidermal Growth-Factor-Containing Fibulin-like Extracellular Matrix Protein 1 Deposition in the Lower Gastrointestinal Tract: An Autopsy-Based Study

    doi: 10.3390/ijms25147581

    Figure Lengend Snippet: Summary of clinical and pathological data in patients with definite or possible constipation.

    Article Snippet: Single IHC was performed using primary antibodies against fibulin-3 (EFEMP1) (mouse, clone mab3-5, 1:2000, Santa Cruz, TX, USA), and phosphorylated α-synuclein (clone LB508, 1:500; Zymed, San Francisco, CA, USA) was performed in all cases.

    Techniques:

    Schematic presentation of the hypothesized pathological effects of EFEMP1/AEFEMP1 deposition in the lower gastrointestinal tract. It is hypothesized that EFEMP1/AEFEMP1 deposition in blood vessels causes impaired blood flow, whereas deposition around the Auerbach plexus, muscularis propria, subserosa, and serosa reduces peristalsis and causes wall vulnerability. Complications such as hypothyroidism, LBD, and diabetes are presumed to be exacerbating factors in these conditions. A combination of these pathologies may result in ischemia of the intestinal tract and associated perforation, ileus, intestinal volvulus, constipation, and diverticulum formation.

    Journal: International Journal of Molecular Sciences

    Article Title: Clinicopathological Appearance of Epidermal Growth-Factor-Containing Fibulin-like Extracellular Matrix Protein 1 Deposition in the Lower Gastrointestinal Tract: An Autopsy-Based Study

    doi: 10.3390/ijms25147581

    Figure Lengend Snippet: Schematic presentation of the hypothesized pathological effects of EFEMP1/AEFEMP1 deposition in the lower gastrointestinal tract. It is hypothesized that EFEMP1/AEFEMP1 deposition in blood vessels causes impaired blood flow, whereas deposition around the Auerbach plexus, muscularis propria, subserosa, and serosa reduces peristalsis and causes wall vulnerability. Complications such as hypothyroidism, LBD, and diabetes are presumed to be exacerbating factors in these conditions. A combination of these pathologies may result in ischemia of the intestinal tract and associated perforation, ileus, intestinal volvulus, constipation, and diverticulum formation.

    Article Snippet: Single IHC was performed using primary antibodies against fibulin-3 (EFEMP1) (mouse, clone mab3-5, 1:2000, Santa Cruz, TX, USA), and phosphorylated α-synuclein (clone LB508, 1:500; Zymed, San Francisco, CA, USA) was performed in all cases.

    Techniques:

    Representative microphotographs of EFEMP1 on elastica–Masson and Congo red staining and IHC for EFEMP1 in the lower gastrointestinal tract. Deposits in the vessel ( a – c ); mucosa and muscularis mucosae ( d – f ); muscularis propria ( g – i ); Auerbach plexus ( j – l ); serosa ( m – o ); and interstitium of the subserosa (including the mesentery) ( p – r ). ( a , d , g , j , m , p ) Elastica–Masson staining; ( b , e , h , k , n , q ) Congo red staining under bright-field illumination; (insets in ( b , n )) Congo red staining under polarized light; ( c , f , i , l , o , r ) IHC for EFEMP1. Notably, areas positive for EFEMP1 deposition show proliferation (panel d ), contortion, thickening, and fragmentation of elastic fibers (panels a , g , j , m , p ). Deposits in the muscularis mucosae show no congophilia ( e ), and deposits in the muscularis propria ( h ), peri-Auerbach plexus ( k ), and interstitium of the subserosa ( q ) exhibit weak congophilia compared with that in the vessel ( b ) and serosa ( n ). Arrowheads indicate EFEMP1 deposits, and the area indicated by the black square is the region shown in the inset. Scale bar = 200 μm ( d – f , g – i ); 100 μm ( a – c , g – i , m – r ).

    Journal: International Journal of Molecular Sciences

    Article Title: Clinicopathological Appearance of Epidermal Growth-Factor-Containing Fibulin-like Extracellular Matrix Protein 1 Deposition in the Lower Gastrointestinal Tract: An Autopsy-Based Study

    doi: 10.3390/ijms25147581

    Figure Lengend Snippet: Representative microphotographs of EFEMP1 on elastica–Masson and Congo red staining and IHC for EFEMP1 in the lower gastrointestinal tract. Deposits in the vessel ( a – c ); mucosa and muscularis mucosae ( d – f ); muscularis propria ( g – i ); Auerbach plexus ( j – l ); serosa ( m – o ); and interstitium of the subserosa (including the mesentery) ( p – r ). ( a , d , g , j , m , p ) Elastica–Masson staining; ( b , e , h , k , n , q ) Congo red staining under bright-field illumination; (insets in ( b , n )) Congo red staining under polarized light; ( c , f , i , l , o , r ) IHC for EFEMP1. Notably, areas positive for EFEMP1 deposition show proliferation (panel d ), contortion, thickening, and fragmentation of elastic fibers (panels a , g , j , m , p ). Deposits in the muscularis mucosae show no congophilia ( e ), and deposits in the muscularis propria ( h ), peri-Auerbach plexus ( k ), and interstitium of the subserosa ( q ) exhibit weak congophilia compared with that in the vessel ( b ) and serosa ( n ). Arrowheads indicate EFEMP1 deposits, and the area indicated by the black square is the region shown in the inset. Scale bar = 200 μm ( d – f , g – i ); 100 μm ( a – c , g – i , m – r ).

    Article Snippet: Single IHC was performed using primary antibodies against fibulin-3 (EFEMP1) (mouse, clone mab3-5, 1:2000, Santa Cruz, TX, USA), and phosphorylated α-synuclein (clone LB508, 1:500; Zymed, San Francisco, CA, USA) was performed in all cases.

    Techniques: Staining

    Representative microphotographs of EFEMP1 deposition grading used in this study: ( a – n ) IHC for EFEMP1; ( a , f , k ) vessel; ( b , g ) muscularis propria; ( c , h , l ) Auerbach plexus; ( d , i , m ) serosa; and ( e , j , n ) mesentery (interstitium). Arrowheads indicate EFEMP1 deposits. Scale bar = 200 μm ( a – n ).

    Journal: International Journal of Molecular Sciences

    Article Title: Clinicopathological Appearance of Epidermal Growth-Factor-Containing Fibulin-like Extracellular Matrix Protein 1 Deposition in the Lower Gastrointestinal Tract: An Autopsy-Based Study

    doi: 10.3390/ijms25147581

    Figure Lengend Snippet: Representative microphotographs of EFEMP1 deposition grading used in this study: ( a – n ) IHC for EFEMP1; ( a , f , k ) vessel; ( b , g ) muscularis propria; ( c , h , l ) Auerbach plexus; ( d , i , m ) serosa; and ( e , j , n ) mesentery (interstitium). Arrowheads indicate EFEMP1 deposits. Scale bar = 200 μm ( a – n ).

    Article Snippet: Single IHC was performed using primary antibodies against fibulin-3 (EFEMP1) (mouse, clone mab3-5, 1:2000, Santa Cruz, TX, USA), and phosphorylated α-synuclein (clone LB508, 1:500; Zymed, San Francisco, CA, USA) was performed in all cases.

    Techniques: